    
ELISA KIT FOR QUANTITATION OF PROTEIN A
This double sandwich ELISA utilizes polyclonal anti-protein A antiserum as a
capture antibody and biotynilated anti-protein A monoclonal antibody and
streptavidin-peroxidase as the detecting antibody.
Each kit contains :
 | A 96 well, 12 strip plate,
pre-coated with a polyclonal capture antibody. |
| Biotinylated, monoclonal
detection antibody. |
| Streptavidin-peroxidase. |
| Protein A standards. |
| A complete set of buffers and
reagents needed to perform an ELISA. |
The assay is :
| Sensitive: detects less than
0.48 ng/ml of protein A. |
| Linear: in the range of
240-3900 pg/ml. |
| Rapid: 3 hours incubation time. |
| Specific: practically
unaffected by the presence of murine immunoglobulins. |
| Reproducible and accurate:
Interassay CV < 10% / Intraassay CV. |
| Stable: over 6 months at 4
degrees C. |
Utility :
| Precise quantitation of
protein A in cell culture media, sera and protein A column eluates. |
| Detection and quantitation of
minute amounts of protein A leaking from the solid supports into elution
buffers during immunoglobulin purification. |
| Detection and quantitation of
protein A in final preparations of murine monoclonal antibodies. |
| Validation and purification
processes for protein A removal from final preparations of immunoglobulins. |
SKU #:76550
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