Biotech research

	Carcinoembryonic antigen
	Monoclonal antibody to carcinoembryonic antigen
	Monoclonal syngeneic anti-idiotypic antibody to anti-carcinoembryonic antigen monoclonal antibody

Mouse monoclonal antibody to carcinoembryonic antigen
TRADE NAME: C₂₃₄

Carcinoembronic antigen (CEA), an oncodevelopmental tumor marker is a fairly typical, high-molecular weight, cell surface glycoprotein over-expressed in adenocarcinomas of entero-dermally-derived digestive system epithelia and in fetal colon.
The majority of antibodies induced against CEA would cross-react with a variety of proteins present in normal tissues such as non-specific cross-reacting antigen (NCA). Highly specific anti-CEA mouse monoclonal antibody was produced as a result of a unique imunization protocol.

ANTIBODY CHARACTERISTICS

Production:

The hybridoma secreting this highly specific anti-CEA monoclonal antibody was obtained from polyethylene glycol fusion of non-secreting mouse myeloma (P3X63.Ag8.653) cells with spleen cells from a BALB/c mice immunized with physically modified CEA.

Purification:

Tissue culture derived antibody was precipitated with ammonium sulfate and chromatographed on protein A-SeparoseB-CL-4B.

Immunologic class :

IgG, k

Isoelectric point :

pH 6.6-6.8

Molecular weight :

165 kd : heavy chain 53 kd
according to B-mercaptoethanol light chain 29.5 kd
reduced SDS-PAGE

Immunologic specificity :

This antibody is CEA specific. It does not recognize NCA, neither in enzyme linked immunosorbent assay (ELISA) not on Western blots of crude tissue extracts.

This antibody binds to CEA producing human adenocarcinoma cells (e.g. LS174T,WiDr) but does not bind to CEA non-secreting human adenocarcinoma cells (Colo 320DM). It does not immunostain cryocuts of any normal human tissues tested (artery, heart muscle, kidney, large bowel, liver, lung, skeletal muscle, skin, small gut, spleen, testes or vein) neither does it react with any antigens on human blood cells when separated by fluoresceine actuated cell sorter (FACS).

Immunohistochemical analysis of cancerous tissues revealed that the antibody bound selectively adenocarcinoma and not carcinoid tumor of the colon, as well as tumor- associated CEA in breast, gastric and lung neoplasms, while there was no immunohistochemical evidence of cross-reactivity with NCA. A remarkable feature of this antibody is its ability to distinguish immunohistochemically between cancer and residual normal tissue within the same organ.

Applications :

	In a sandwich quantitative ELISA for CEA. It detects CEA in the range 1-40 ng/ml.
	For purification of CEA by affinity chromatography after coupling it to CNBr activated Sepharose 4B. Such and immunosorbent retained only CEA from the perchloric acid (PCA) extract of hepatic metastases from colorectal cancers.
	For immunohistochemical differentiation between neoplasmic and healthy tissues.
	It seems to be an ideal candidate for "in vivo" tumor imaging by immunoscintigraphy.

Storage :

Store at 4^oC.

Stability :

When reconstituted in aqueous solution IgG remains stable for six months if stored at 4^oC. No considerable loss of activity was detected when stored at -20^oC over a period of six months.

Delivery form :

Lyophilized pure IgG

Delivery quantities :

Monoclonal antibody to CEA C234 IgG
                - 1 mg/vial SKU #: 76050
                - 1 mg/vial SKU #: 76055
                - 1 mg/vial SKU #: 76060

Monoclonal antibody to CEA C₂₃₄ F(ab'₂)
                - 1 mg/vial SKU #: 76100
                - 1 mg/vial SKU #: 76105
                - 1 mg/vial SKU #: 76110