| Immunoglobulin
class : |
IgG1,
k
|
| Isoelectric
point : |
pH
7.35-6.55
|
| Molecular
weight of IgG : |
according
to SDS-PAGE under mercaptoethanol 153.8 kd 49.7 kd heavy chain
reduced conditions: 27.2 kd light chain.
|
| Molecular
weight of F(ab')2 : |
according
to SDS-PAGE under mercaptoethanol 116 kd 30.8 kd heavy chain
reduced conditions: 27.2 kd light chain.
|
| Antibody
production : |
Clinical
grade tissue culture supernatant was concentrated and purified to
homogeneity by a series of chromatographic steps. Immunoglobulin was then
fragmented to F(ab')2 by proteolytic degradation and reduced to Fab' by
cysteine.
|
| Kit
preparation : |
Pure
3-48 Fab' fragments were "pre-tinned", excess tin removed and
antibody fragments fromulated in the presence of technetium reducing agent
into sterile hypovials, and lyophilized.
For radiolabelling, vial content is reconstituted with 99mTc
freshly eluted from the generator and after 30 minute incubation at room
temperature is ready for i.v. injection.
|
| Affinity
constants for : |
Antigen |
|
IgG |
Fab' |
|
Human
ventricular myosin heavy chains |
3.33
x 108M-1 |
|
|
Human
atrial myosin heavy chains |
1.48
x 108M-1 |
|
|
Human
skeletal muscle myosin |
1.06
x 108M-1 |
|
|
Canine
cardiac myosin heavy chains
|
2.06
x 108M-1 |
1.2
x 107M-1 |
| Immunologic
specificity : |
The
monoclonal antibody is myosin alpha and beta heavy chain specific. On
Western blots and in ELISA it reacts with purified heavy chains of atrial
and ventricular myosin isolated from human, canine, bovine, porcine,
murine, rabbit, and rat cardiac tissues. It binds to A-band of thick
filament of cardiac and skeletal muscle myosin as detrmined by indirect
immunofluorescence on cryocuts, and indirect immunoperoxidase staining of
Carnoy fixed tissues. It stains human and canine myocytes in culture as
determined by indirect immunofluorescence.
|
| Cross-reactivity
: |
3-48
antibody cross-reacts with heavy chains of human myosin isolated from:
slow skeletal muscle, fast skeletal muscle, eye muscle, and streptococcal
M5 peptide. It does not react with human platelet, smooth muscle myosin
heavy chains, myosin light chains, myosin fragments circulating in blood
of patients with myocardial infarction, nor does it react with any other
human tissue antigens or blood cells.
|
| Storage
: |
Store
at 4oC.
|
| Stability
: |
IgG |
When
reconstituted in aqueous solution IgG remains stable for six months if
stored at 4oC. No considerable loss of activity was detected
when stored at -70oC over a period of two years. |
|
F(ab')2 |
When
reconstituted in aqueous solution F(ab')2 remains stable for
six months
if stored at 4oC. No considerable loss of activity was detected
when stored at -20oC over a period of one year. |
|
CVTM |
Stable
for one year when stored lyophilized at 4oC.
|
| Delivery
form : |
Lyophilized
|
| Delivery
quantities : |
-
1 mg/vial SKU #: 76250
- 5 mg/vial SKU #: 76255
|
